PNC-27
What it’s used for
Lab & animal research
PNC-27 is an experimental peptide built from part of the p53 protein. It targets HDM-2, a protein on cancer-cell membranes, and in lab dishes it has killed cancer cells while sparing some normal cells. There are no human trials, and the FDA found bacterial contamination in a marketed sample.
- Cancer-cell killing Killed cervical, leukemia and ovarian cancer cells in lab studies by forming pores in their membranes.
- Spares some normal cells In lab tests it left normal cervical cells and normal blood-forming cells unharmed.
- Chemotherapy pairing With paclitaxel, it showed an added effect in tissue samples tested outside the body.
Side effects & safety
Side effects
No side effects documented yet.
Stop and get help if
- Fever or chills
- Infected injection sites
- Signs of an allergic reaction
- Feeling generally unwell
- Worsening cancer symptoms — get a medical assessment
Which symptoms mean call 911 or see a doctor
What regulators say
- In January 2017 the FDA found a marketed PNC-27 sample contaminated with the bacterium Variovorax paradoxus; that's one product, not a rate for all.
Why most peptides aren’t FDA-approved What’s really in “research use only” vials
Avoid or check first if
- Using it in place of cancer treatment — there's no human evidence it works or is safe
In sport
Likely banned at all times. An experimental anticancer peptide with no approval or registered trials, so it’s an S0 substance.
Which peptides are banned in sport
How much people take
For every goal: Once daily · under the skin
- Standard research protocol Abdomen 100–500 mcg
- Starting & conservative dose 100 mcg
Similar doses appear in 1 other source
How to mix and store it
How many units do I draw?
How to mix 5 steps with bacteriostatic water
How to mix
- 1 Wipe both tops Wash your hands, then swab the rubber tops of the peptide vial and the water vial with alcohol. Let them dry.
- 2 Draw the water Use bacteriostatic (BAC) water — sterile water with a preservative, sold for mixing peptides . With a new sterile syringe, draw the amount your product calls for. A 1 mL syringe filled twice is 2 mL.
- 3 Run it down the wall Push the needle into the peptide vial at an angle and let the liquid trickle down the glass, not onto the powder.
- 4 Swirl, don’t shake Gently swirl or roll the vial until the liquid is completely clear. Give it a few minutes if needed.
- 5 Label the vial Write the date and what you mixed (for example 5 mg + 2 mL) on the vial.
This is general practice. If your product came with its own diluent or instructions, follow those. Not sure what to buy? See the supplies checklist .
Injecting under the skin Where, rotating sites, which syringe
- Where: belly (at least 2 in / 5 cm from the navel), front of the thigh, or back of the upper arm.
- Rotate sites each time and skip bruised, scarred or sore skin.
- Syringe: most people use a U-100 insulin syringe with a short, thin needle (29–31 gauge, 8 mm or shorter).
- How: wipe with alcohol and let it dry, pinch the skin, go in at 45–90°, push slowly. Use each syringe once.
General practice for under-the-skin injections, not specific to PNC-27.
The full guide: how to inject safely
Storage
Fridge once mixed Mixed vial at 2–8°C (35.6–46.4°F); one source gives 28 days. Dry powder at -20°C (-4°F) to -80°C (-112°F)
Combining it with others
AI assessment Based on how each compound works, not on studies of the combination. How we assess
Use caution 3
Can be combined 2
What the research says
Mostly animal studies
- No human trial shows a cancer benefit, longer survival, a safe dose or whole-body safety.
- Sparing one normal cell line in a dish doesn't show normal organs are safe in the body.
- Cancer cells died within 90 minutes in the lab; that isn't a timeline for patients.
- The research doesn't confirm the identity, purity or handling of products bought separately.
- Inhaled or nebulized products, and products sold with cure claims, are red flags.
- Human half-life is unknown, and the 28-day mixed-vial figure hasn't been confirmed.
- No controlled interaction study exists; missing data doesn't mean combinations are safe.
What studies found
- Cervical cancer cells in culture The study tested HTB-35/SiHa and a normal cervical-cell line. Its reported 12.4 μM IC50 is a cell-culture concentration, not a human dose.
- FDA: Unproven products marketed for cancer FDA identifies a January 2017 PNC-27 sample contaminated with Variovorax paradoxus; this is a specific product finding, not an incidence estimate for all products.
A half-life hasn’t been verified for this compound.
What it’s made of
- Length
- 32 amino acids
- Molecular weight
- 4031.72 Da
- Formula
- Not verified
An exact sequence has not been verified for this form. Reported length: 32 residues.
Sequence and formulation must be confirmed for the exact material; similarly named products are not necessarily interchangeable.
Length and weight come from a reference listing and were not independently confirmed.
Sources
Sources 12
How this page was made
How it may work: Targets membrane HDM-2 and disrupts membranes; mitochondrial effects are also investigated.
Other names: PNC27
Preparation in studies: A product-specific preparation or sterility protocol has not been verified.
Reported selectivity does not establish whole-body safety.
Research last checked: Oct 12, 2026 (a targeted search, not a full review).
Community doses, how long people take it, timing and storage are compiled with AI from public peptide guides, clinics and forums, cross-checked against other sources where we could, and rewritten in plain language. “Combining it with others” is an AI assessment of how the compounds work. Study notes are written from each paper’s abstract where one exists. How we source
Original source guide